The announcement arrives as the studio prepares for the game's December 10 release, which was reconfirmed during Nintendo's September Direct.
Pre-orders for PC and PlayStation 5 are live now, with Nintendo Switch pre-orders expected at a later date.
Developer Level-5 published the English-language cast list on the game's official site and included short audio snippets that showcase the returning North American voices for Professor Layton and Luke.
Christopher Robin Miller and Lani Minella, who voiced Layton and Luke respectively in earlier series entries, are credited as leads for the new title.
The wider English cast includes Amber Lee Connors (credited on the site for roles such as Persona 4 Revival and Unicorn Overlord), Nic Olsen (Genshin Impact), and Arthur Romeo (Street Fighter 6, AI: The Somnium Files - nirvanA Initiative).
The UK dub lists Cat Protano in the role of Luke, a change from the previous main-series UK actor Maria Darling.
Level-5 also confirmed that the French, Italian, German and Spanish dubs employ professional voice actors rather than synthetic voices.
Paraphrasing the studio’s messaging, Level-5 made clear that regional recordings use human performers for all spoken dialogue in those releases.
The developer’s statement about AI usage was reiterated alongside the cast reveal.
Level-5 CEO Akihiro Hino has stated that artificial intelligence tools were not used in the game's voice work or wider production.
That assertion contrasts with the increasingly common industry practice of disclosing AI-assisted assets; notably, the game's newly published Steam page does not include an AI disclosure banner required for titles that use in-game AI-generated assets.
The absence of that disclosure on Steam does not, by itself, indicate whether AI was used, but Level-5’s public comment maintains that no AI was employed.
With pre-orders open for PC and PS5 and a firm December 10 release date set during the Nintendo Direct, fans of the Layton series have a clearer picture of what to expect at launch.
The return of familiar voice actors will likely be a welcome sign for longtime players watching Level-5’s revival of the franchise on Nintendo Switch, PC, and PlayStation 5.